Induction and Phytochemical Analyses in <i>Stryphnodendron adstringens</i> (Mart.) Coville Calli

Abstract

Stryphnodendron adstringens is a medicinal plant considered as endangered species due to the destructive methods used for tannins extraction. The  objective of this study was to induce calli development from nodal segments of Stryphnodendron adstringens (Mart.) Coville and to evaluate the total phenol and tannin contents of the induced calli. Results indicated that calli induced in the presence of 0.5 and 2.0 mg L^-1 picloran or picloran associated with 0.1 mg L^-1 kinetin, showed higher fresh matter values (0.120 g and 0.116 g, respectively). The highest total phenol contents were obtained in calli grown in the absence of growth regulators (9.58%) and in the presence of 0.1 mg L^-1 kinetin (9.23 %). Highest yields of total tannins was observed in calli induced in the absence of growth regulators (2.36%) and in the presence of 2.0 mg L^-1 picloran (1.71%).