Use of micropropagation in the vegetative rescue of adult trees of Cedrela odorata L.

Abstract

The objective of this work was the vegetative rescue of Cedrela odorata L. adult trees from forest areas by micropropagation using shoot regeneration from axillary buds in vitro. Nodal segments (0.5 and 1.0 cm) of shoots formed from cuttings taken from canopy sprouts were used. The explants were successfully established in vitro on MS medium supplemented with 2.22 ?M BAP, 0.49 ?M AIB, 0.28 ?M GA3 and PPM (0 and 8.6 ?M). Shoot multiplication on MS with combinations of GA3 (0.28 and 1.12 ?M) and BAP (2.2, 3.3, 4.4, 6.6, and 8.8 ?M) and shoot rooting on medium with 4.9 ?M AIB, 5.7 ?M AIA or 5.37 ?M ANA were evaluated. The MS medium supplemented with 0.28 ?M GA3 and 8.8 ?M BAP generated 4.16 shoots per explant. The best rooting induction was observed on medium containing 4.9 ?M IBA, resulting in 60% of shoot rooting. The plantlets rooted were acclimatized and showed normal development with 97% survival rate. The use of canopy sprouts as explants is feasible in the rescue of C. odorata and the combination of BAP and GA3 favors in vitro multiplication.