Free proline and total flavonoids from Lippia origanoides submited to base saturation levels and water stress

Abstract

We aimed to evaluate the behavior of the accesses of rosemary pepper (Lippia origanoides) at different levels of saturation and water stress from the level of free proline, total flavonoids and silicon accumulation. The experiment consisted of accesses submitted to saturation levels with agrosilicon (0.92; 2.47; 4.0; 5.55 e 7.09 g of Si) which were evaluated during daily irrigation and after 10 days of water suspension. The amount of proline was obtained by means of sulfosalicilic acid and acid ninhydrin, the flavonoid contents was obtained by means of metanolic extract and aluminum chloride and the silicon contents by means of molybdenum blue. The absorbances were recorded at 520 nm for proline, 425 nm for flavonoid and 660 nm for silicon. The results were submitted to analysis of variance and regression. The best dose response of silicon between the accesses was of 20 to 50 %. The ICA-3 and ICA-4were those that presented the best production for the two variables, with a capacity to accumulate up to ± 3.0 ?mol g-1 of the proline in fresh matter and up to ± 250 RE g-1 of flavonoids content in dry mass. ICA- 3 access presented better silicon accumulation in leaves. Thus, the addition of 2.17 t ha-1 of agrosilicic in cultivation of Lippia origanoides favors the production of secondary metabolites under water stress.