Technological and antimicrobial properties of fish protein biofilms with clove essential oil

Abstract

The aim of this work was to evaluate the antimicrobial capacity of biofilms, made with protein obtained from filleting residues of dourada (Brachyplatysoma rousseauxii) added of clove essential oil (Eugenia caryophyllata), and to analyze the technological properties. Two biofilms were elaborated, one with 2% myofibrillar proteins extracted from filleting shavings and the other one with 2% dourada skin gelatin, by the casting method. The clove essential oil concentrations were 0, 1, 1.5 and 2% in relation to the volume of the solution. The antimicrobial activity was evaluated by the disk diffusion method and mechanical analyses as barrier, thickness, color and morphological and structure characteristics by the scanning electron microscopy were performed. The biofilms added of clove essential oil presented excellent values of water vapor permeability and high values of elongation, contributing for the better application in foods, as well as they presented yellow color with low intensity. The biofilms with myofribillar proteins presented inhibitory effect in the concentrations used against the Gram positive bacteria, Staphylococus aureus and Enterococus fecalis. The gelatin biofilms added of clove essential oil did not inhibit the growth of Gram positive and Gram negative bacteria.